Pest Management Science (2007) 63, 68-74
Hui-Fang Guo, Ji-Chao Fang, Bao-Sheng Liu, Jie-Ping Wang, Wan-Fang Zhong and Fang-Hao Wan (2007)
Enhancement of the biological activity of nucleopolyhedrovirus through disruption of the peritrophic matrix of insect larvae by chlorfluazuron
Pest Management Science 63 (1), 68-74
Abstract: The enhancement of Spodoptera litura (F.) nucleopolyhedrovirus (SlNPV) activity using the chitin synthesis inhibitor chlorfluazuron was investigated. When tested against fifth-instar S. litura larvae, chlorfluazuron produced synergistic effects at doses of 0.05 and 0.025 µg per insect, and additive effects at doses of 0.1 and 0.2 µg. Furthermore, the time required for SlNPV to kill larvae was significantly reduced by chlorfluazuron at all doses tested. The activity and killing speed of Autographa californica (Spey) nucleopolyhedrovirus (AcMNPV) against third-instar Spodoptera exigua (Hübner) larvae were similarly improved by chlorfluazuron at a dose of 0.05 µg per larva. Furthermore, the growth of S. exigua was significantly retarded by chlorfluazuron. Environmental scanning electron microscopy (ESEM) showed that the peritrophic matrices (PMs) of S. litura exposed to chlorfluazuron alone, or the combination treatment, were markedly disrupted. Obvious ruptures on the outer surfaces of the PM were observed, which potentially facilitated the passage of virions through the matrix.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jichao Fang
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
formulation/storage of bioagents
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Spodoptera exigua | ||||
| Spodoptera litura | ||||
| Autographa californica nucleopolyhedrovirus (entomopathogen) | Spodoptera exigua | |||
| Spodoptera litura nucleopolyhedrovirus (entomopathogen) | Spodoptera litura |
