View source for Phytopathology (1996) 86, p. S99 (Mahmood et al.)

{{Publication
|Publication authors=T. Mahmood and G.L. Hein
|Publication date=1996
|dc:title=Identification of double infection of wheat with high plains disease and [[Wheat streak mosaic virus]] from wheat curl mites in western Nebraska
|Publication journal=Phytopathology
|prism:volume=86 (11 suppl.)
|prism:startingPage=S99
|Last page=S99
|Publication abstract=A new disease of wheat and corn has been observed in the high plains region. The causal agent of this disease, commonly called high plains disease (HPD), most likely is a virus. HPD is transmitted by the wheat curl mite, ''Aceria tosichella'' Keifer, the same eriophyid mite vector of wheat streak mosaic virus (WSMV). In the field it is extremely difficult to visually differentiate plants infected with WSMV from those with HPD. An indirect protein-A sandwich ELISA (PAS-ELISA), and western blot analysis were used to detect WSMV and HPD agent. Random samples of wheat curl mites from western Nebraska commercial wheat plantings were collected and used to infest caged wheat plants. After three weeks, leaf samples were harvested and assayed. Of 400 samples tested by western blot analysis, 73%, 49% and 43% were positive for WSMV, HPD pathogen, or both, respectively. Samples tested by PAS-ELISA were 54%, 36% and 28% positive for WSMV HPD pathogen, or both, respectively. These data indicate and unexpectedly high incidence of HPD in the mite population sampled.
|AbstractCC=No
|Research topic=identification/taxonomy
|Research2P=transmission/dispersal of plant diseases
|Is book=No
}}
{{Pest record
|Pest=Emaravirus tritici
|Crop=Wheat (Triticum)
|Country=U.S.A. (mid N)
|Quarantined=No
}}
{{Pest record
|Pest=Wheat streak mosaic virus
|Crop=Wheat (Triticum)
|Country=U.S.A. (mid N)
|Quarantined=No
}}