Turkish Journal of Agriculture and Forestry (2007) 31, 41-46

Harun Bayraktar, F. Sara Dolar and Salih Maden (2007)
Mating type groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the causal agent of chickpea blight in Central Anatolia
Turkish Journal of Agriculture and Forestry 31 (1), 41-46
Abstract: The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 (57.8%) and the others (42.2%) to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods.
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Database assignments for author(s): Fatma S. Dolar, Salih Maden

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ascochyta rabiei	Chickpea (Cicer arietinum)	Turkey