Tropical Plant Pathology (2021) 46, 386-391

Cristiane Müller, Mariana Bossi Esteves, Heloisa Thomazi Kleina, Aline Nondillo, Marcos Botton and João Roberto Spotti Lopes (2021)
First sharpshooter species proven as vectors of Xylella fastidiosa subsp. multiplex in Prunus salicina trees in Brazil
Tropical Plant Pathology 46 (3), 386-391
Abstract: Plum leaf scald (PLS), caused by the vector-borne bacterium Xylella fastidiosa, is a major obstacle to the expansion of plum crops in Brazil. In other affected crops, this pathogen is naturally transmitted mainly by sharpshooter leafhoppers (Hemiptera: Cicadellidae: Cicadellinae). Despite the importance of PLS, the vector species responsible for bacterial spread in plums remain unknown. This study, therefore, aimed to determine the X. fastidiosa transmission ability of three sharpshooter species commonly found in plum orchards in southern and southeastern Brazil: Macugonalia cavifrons (Stål), Macugonalia leucomelas (Walker), and Sibovia sagata (Signoret). After a 72-h acquisition access period to plum trees infected with X. fastidiosa subsp. multiplex (sequence type 67), the insects were transferred for an inoculation access period of 96 h to 10–20 healthy Prunus salicina plants for each sharpshooter species, using four insects per plant. Bacterial infection in the plants was verified 7 months after inoculation by polymerase chain reaction. Results showed that the three sharpshooter species were able to transmit X. fastidiosa to plums, with mean transmission efficiencies by single insects of 16%, 14%, and 18% for M. cavifrons, M. leucomelas, and S. sagata, respectively. To our knowledge, this study is the first to identify vector species and prove the transmission of a PLS strain of X. fastidiosa subsp. multiplex by sharpshooters in Brazil.
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Database assignments for author(s): Marcos Botton, João Roberto Spotti Lopes

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xylella fastidiosa	Prunus (other fruit tree species)	Brazil (south)
	Macugonalia cavifrons	Prunus (other fruit tree species)	Brazil (south)
	Macugonalia leucomelas	Prunus (other fruit tree species)	Brazil (south)
	Sibovia sagata	Prunus (other fruit tree species)	Brazil (south)