Tropical Plant Pathology (2020) 45, 597-610

Jefferson Rangel da Silva, Fausto Veiga de Alvarenga, Rodrigo Marcelli Boaretto, João Roberto Spotti Lopes, José Antônio Quaggio, Helvecio Della Coletta Filho and Dirceu Mattos Jr (2020)
Following the effects of micronutrient supply in HLB-infected trees: plant responses and ' Candidatus Liberibacter asiaticus' acquisition by the Asian citrus psyllid
Tropical Plant Pathology 45 (6), 597-610
Abstract: Despite efforts of research to establish best nutrient management practices in HLB-affected citrus orchards, there are still doubts about the contribution of such strategies to minimizing losses caused by the disease in the citrus industry. We evaluated the effects of micronutrient (Zn, Mn, and/or Cu) supply and ' Candidatus Liberibacter asiaticus' (CLas) infection on physiological and growth traits of sweet orange trees (Citrus sinensis (L.) Osbeck)) to understand if enhanced micronutrient supply would improve the growth of plants infected with CLas and reduce bacterial infection, as well as the acquisition of CLas by adults and nymphs of Diaphorina citri, correlated with nutritional status of trees. Plants were either grafted with buds obtained from micrografted plants (healthy, -) or buds infected with CLas (diseased plants, +). Infected plants were exposed to one of the five nutrient treatments, applied to leaf and root: (i) nilZnMnCu+: not fertilized with Cu, Mn, and Zn; (ii) Zn+: fertilized with zinc sulfate (ZnSO₄.H₂O); (iii) Mn+: fertilized with manganese sulfate (MnSO₄.H₂O); (iv) Cu+: fertilized with copper hydroxide [Cu(OH)₂]; and (v) ZnMnCu+: fertilized with all three micronutrients. Likewise, healthy plants were exposed to one of the two treatments as above: (i) nilZnMnCu- or (v) ZnMnCu-. We found that CLas impairs plant biomass production regardless of nutrient treatments, especially root growth, and increases specific leaf dry weight as disease progresses because of starch accumulation. Moreover, individual supply with Zn, Mn, or Cu can mitigate such deleterious effects of HLB on starch metabolism. HLB also changes nutrient concentrations in both leaves and sap extract, regardless of nutrient treatments, although treatments do not reduce the CLas titer in plants as determined by RT-qPCR. On the contrary, micronutrients applied in combination (ZnMnCu+) can reduce the acquisition of CLas by adults and especially nymphs of D. citri, likely reducing the disease infection in citrus orchards.
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Database assignments for author(s): João Roberto Spotti Lopes

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases
environment - cropping system/rotation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liberibacter asiaticus	Citrus (genus)
	Diaphorina citri	Citrus (genus)