Tropical Plant Pathology (2019) 44, 343-351

Ashish Kumar Nayak and Kishore Babu Bandamaravuri (2019)
Detection of Golovinomyces orontii using species-specific primers and high-resolution melting analysis
Tropical Plant Pathology 44 (4), 343-351
Abstract: This study presents the development of species-specific PCR primers for detection of Golovinomyces orontii, the causal agent of powdery mildew disease on different cucurbit hosts. The species-specific primers CgF2 and CgR2 were designed based on partial ITS and 5.8S rDNA sequences using an isolates collection of powdery mildew and downy mildew pathogens from different sources. After optimization of the PCR conditions, a 233 bp fragment was amplified only from DNA of G. orontii. Limit of detection was determined to be 0.01 ng of pure conidial genomic DNA. Further association of PCR and high-resolution melting (HRM) analysis showed that the designed primer set can be used for differentiation of G. orontii from different powdery mildew fungi in infected cucurbits tissue collected in the field.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Golovinomyces orontii