Tropical Plant Pathology (2019) 44, 302-307

Guo-jun Hu, Guo-ping Wang and Ni Hong (2019)
Effect of in vitro sub-culture of sandy pear on variant structure of Apple stem grooving virus
Tropical Plant Pathology 44 (3), 302-307
Abstract: In vitro culture of sand pear (Pyrus pyrifolia) cv. Huanghua (HH) infected with apple stem grooving virus (ASGV) was conducted and the titer and variant mutations of the virus in sub-culture were analyzed. The results showed that ASGV could replicate stably in 20 successive sub-cultures. The 500 bp PCR products of ASGV in each sub-culture were cloned and used for single-strand conformation polymorphism analysis. The results demonstrated that there was a dominant molecular variant of ASGV in vitro and that the variant structure of ASGV is stable in sub-culturing. The rate of the main haplotype in the 20 passages was from 78.0% to 95.7%. To clarify the origin of subordinate haplotypes, the error rate of our RT-PCR assay (5.4× 10^-4/nt) was computed and the results indicated that the mutations which appeared in sub-passages were acquired from artificial operation and not produced by viral replication.
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Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Apple stem grooving virus