Tropical Plant Pathology (2019) 44, 238-243

Ayaka Uke, Sophary Khin, Kengo Kitaura, Masashi Ugaki and Keiko T. Natsuaki (2019)
Combination of an image-posting system and molecular diagnosis for detecting Sri Lankan cassava mosaic virus
Tropical Plant Pathology 44 (3), 238-243
Abstract: Cassava mosaic disease (CMD), a serious threat to cassava production, is caused by two begomovirus species in Asia, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). Cassava mosaic disease has recently spread into Southeast Asia and it is urgent to establish an efficient system to monitor its spread. Cassava fields were surveyed for CMD on the web using a smartphone-based farming support application, AGRIBUDDY, to obtain photos of CMD and their time, location, and contact information posted to the AGRIBUDDY cloud by farmers. To diagnose a pathogen, we designed novel PCR primers for CMD identification. By combining these two techniques, we detected SLCMV occurrence in Kampong Thom, Cambodia.
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Database assignments for author(s): Keiko T. Natsuaki

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sri Lankan cassava mosaic virus		Cambodia