Theoretical and Applied Genetics (2013) 126, 1201-1212

Thomas Lüpken, Nils Stein, Dragan Perovic, Antje Habekuß, Ilona Krämer, Urs Hähnel, Burkhard Steuernagel, Uwe Scholz, Rounan Zhou, Ruvini Ariyadasa, Stefan Taudien, Matthias Platzer, Mihaela Martis, Klaus Mayer, Wolfgang Friedt and Frank Ordon (2013)
Genomics-based high-resolution mapping of the BaMMV/BaYMV resistance gene rym11 in barley (Hordeum vulgare L.)
Theoretical and Applied Genetics 126 (5), 1201-1212
Abstract: Soil-borne barley yellow mosaic virus disease, caused by different strains of Barley yellow mosaic virus (BaYMV) and Barley mild mosaic virus (BaMMV), is one of the most important diseases of winter barley (Hordeum vulgare L.) in Europe and East Asia. The recessive resistance gene rym11 located in the centromeric region of chromosome 4HL is effective against all so far known strains of BaMMV and BaYMV in Germany. In order to isolate this gene, a high-resolution mapping population (10,204 meiotic events) has been constructed. F2 plants were screened with co-dominant flanking markers and segmental recombinant inbred lines (RILs) were tested for resistance to BaMMV under growth chamber and field conditions. Tightly linked markers were developed by exploiting (1) publicly available barley EST sequences, (2) employing barley synteny to rice, Brachypodium distachyon and sorghum and (3) using next-generation sequencing data of barley. Using this approach, the genetic interval was efficiently narrowed down from the initial 10.72 % recombination to 0.074 % recombination. A marker co-segregating with rym11 was developed providing the basis for gene isolation and efficient marker-assisted selection.
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Link to article at publishers website
Database assignments for author(s): Frank Ordon, Dragan Perovic

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Barley mild mosaic virus	Barley (Hordeum vulgare)	Germany
	Barley yellow mosaic virus	Barley (Hordeum vulgare)	Germany