Scientia Agricola (2007) 64, 482-485

Vicente Savonitti Miranda, Paulo Roberto Silva Farias, Sérgio Rufo Roberto and Pedro Magalhães Lacava (2007)
Genetic characterization of Xylella fastidiosa isolated from citrus and coffee plants
Scientia Agricola 64 (5), 482-485
Abstract: The Citrus Variegated Chlorosis and the Coffee Leaf Scorch are some of the many destructive diseases caused by Xylella fastidiosa, a gram-negative bacterium limited to the xylem of affected plants. As its genetic characterization is still not well established, different isolates of X. fastidiosa from citrus and coffee were evaluated through RAPD (Random Amplified Polymorphic DNA) technique to characterize and classify these isolates based on similarity coefficients. Sixteen isolates of X. fastidiosa were used on this trial, obtained from citrus, coffee and almond. The genetic polymorphism evaluation was performed using six arbitrary 10-base primer pairs. It was possible to establish a dendogram in which the isolates were classified into five groups (A, B, C, D and E). A prevalence of citrus isolates in groups A and D was observed. In groups B and C, there was a prevalence of coffee isolates meanwhile the group D consisted of the almond isolate, solely.
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Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xylella fastidiosa	Almond (Prunus dulcis)	U.S.A. (SW)
	Xylella fastidiosa	Citrus (genus)	Argentina
	Xylella fastidiosa	Citrus (genus)	Brazil (south)
	Xylella fastidiosa	Coffee (Coffea)	Brazil (south)