Revista Brasileira de Fruticultura (2013) 35, 316-319

Adriano Márcio Freire Silva, Natoniel Franklin de Melo, Elineide Barbosa de Souza, Ângela Katiusia Coelho and Rosa de Lima Ramos Mariano (2013)
Limpeza clonal de mudas de videira infectadas por Xanthomonas campestris pv. viticola
[Clonal cleaning of grapevine plants infected by Xanthomonas campestris pv. viticola]
Revista Brasileira de Fruticultura 35 (1), 316-319
Abstract: Bacterial canker is caused by Xanthomonas campestris pv. viticola (Xcv). In order to eliminate Xcv from 'Red Globe' plants it was studied: optimal size of meristem tips and axillary buds for cultivation in modified Galzy's medium (MGM); effects of thermotherapy (38ºC/30 days); and action of antibiotics in the elimination of Xcv in infected grapevines. The percentages of contamination by Xcv and regeneration were analyzed and plants obtained were indexed using the semi-selective culture medium nutrient agar-dextrose-yeast extract-ampicilin (NYDAM) followed by a pathogenicity test. The cultivation of 3 mm explants permitted to obtain plants free of bacteria with regeneration 14.3 times higher than 1 mm explants. The thermotherapy of infected plants associated to the in vitro culture did not eliminate the pathogen. The cultivation of 10 mm explants during 40 days in MGM + cefotaxime (300 mg L^-1) eliminated Xcv from grapevine plants. The indexation of micropropagated grapevine plants for Xcv infection by using NYDAM medium followed by a pathogenicity test is an economical and efficient alternative to produce plants of high sanity quality.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Portuguese)
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Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas citri pv. viticola