Polish Journal of Entomology (Polskie Pismo Entomologiczne) (2014) 83, 281-294

Majid Kazzazi, Fahimeh Dehghanikhah, Hossein Madadi and Vahid Hossseininaveh (2014)
Biochemical characterisation of alpha-glucosidase and ß-glucosidase in the alimentary canal of larval Leptinotarsa decemlineata SAY, 1824 (Coleoptera: Chrysomelidae)
Polish Journal of Entomology (Polskie Pismo Entomologiczne) 83 (4), 281-294
Abstract: Host plant resistance is an environmentally safe method used for reducing a pest population. Basically, when developing resistant cultivars one needs to study the biochemical characteristics of the digestive enzymes in the insect's midgut. In this study, the activities of alpha- and ß-glucosidase were determined from Leptinotarsa decemlineata midgut using p-nitrophenyl-alpha-Dglucopyranoside and p-nitrophenyl-ß-D-glucopyranoside as substrates respectively. The results showed that the specific activity of alpha- and ß-glucosidase from 4th instar larvae midguts of L. decemlineata were 5.14 and 5.48 Umg^-1 protein respectively. The activity of alpha-glucosidase was optimal at pH 4, whereas the maximum activity of ß-glucosidase in the midgut of L. decemlineata occurred at pH 4-5.5. Both enzymes were stable at pH 3-8 over an incubation time of 8 hours. The respective activities of alpha- and ß-glucosidase were at their highest at 45 °C and 50 °C, but they were not stable at 50 °C during an incubation time of 8 days. Furthermore, our data showed that MgCl₂, Tris and urea have a moderate but SDS a severe inhibitory effect on enzyme activity. Biochemical characterisation revealed one and three bands of alpha- and ß-glucosidase activities in the midgut of L. decemlineata respectively.
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Database assignments for author(s): Hossein Madadi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Leptinotarsa decemlineata		Iran