Plant Protection Bulletin (Taipei) (2006) 48, 17-30
W.R. Chen, G.C. Li, S.S. Kao, Y.M. Tzeng and C.C. Tzeng (2006)
Cloning and expression of the insecticidal crystal protein gene, cry 1Ac 5, of Bacillus thuringiensis D4-01 from Taiwan
Plant Protection Bulletin (Taipei) 48 (1), 17-30
Abstract: A new cry -type gene ( cry 1Ac 5 ) was cloned and sequenced from Bacillus thuringiensis ( Bt ) D4-01 native to Taiwan . The full-length (3.7-kb) nucleotide sequence of the cry 1Ac gene for insecticidal crystal protein (ICP) was amplified from Bt isolate D4-01 by PCR. This cry 1Ac gene was constructed in the Escherichia coli plasmid vector, yTandA. After transformation, the cry gene carried by the pSB909.5 expression vector was electroporated into Bt Cry - B (a crystal-negative host), and the recombinant Bt expressing a 130-kDa ICP was obtained. The sequence of 1Ac 5SB cloned from D4-01 was determined. The new gene, cry 1Ac 5 , consisted of an open reading frame of 3.7-kb, which encoded 1177 amino acid residues. The polypeptide has the deduced amino acid sequences predicting a molecular mass of 130-kDa. The cry 1Ac 5 gene had 99.9% homology with the previously reported cry 1Ac 5 gene, and only 2 mistakes were found in comparison to a sequence stored in GenBank. The mortality of the Bt Cry - B clone 1Ac 5SB against larvae of Trichoplusia ni after 72 h of incubation was 60%~80% when treated with 11.75 ppm crystal protein. The mortality of the Bt Cry - B clone 1Ac 5SB against larvae of Plutella xylostella after 72 h of incubation was 80%~90% when treated with 11.75 ppm crystal protein.
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Database assignments for author(s): Suey-Sheng Kao
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Bacillus thuringiensis Cry1A-toxin (entomopathogen) | Taiwan |
