Plant Pathology (2020) 69, 901-910

WanXue Bao, Takuya Nagasaka, Shin Inagaki, Sho Tatebayashi, Iori Imazaki, Shin-ichi Fuji, Takashi Tsuge, Masafumi Shimizu, Koji Kageyama and Haruhisa Suga (2020)
A single gene transfer of gibberellin biosynthesis gene cluster increases gibberellin production in a Fusarium fujikuroi strain with gibberellin low producibility
Plant Pathology 69 (5), 901-910
Abstract: Fusarium fujikuroi , the causative agent of bakanae disease in rice, produces many kinds of secondary metabolites. Recently, two phylogenetic subgroups (F and G groups) of Japanese F . fujikuroi have been identified and found to have differences in their gibberellin (GA) and fumonisin production. G-group F . fujikuroi produces large amounts of GA, but is a fumonisin nonproducer. F-group produces large amounts of fumonisin, but is a GA low or nonproducer. We investigated the cause of low GA production in the F-group. Genetic mapping suggests that low GA production in the F-group strain Gfc0825009 is due to a GA gene cluster for GA biosynthesis. Analysis of the nucleotide and amino acid sequences of the genes in the GA gene cluster showed >98.4% homology between the F-group strain Gfc0825009 and the G-group strain Gfc0801001. Following a 7-day culture under low nitrogen conditions, we found that expression of P450-1 , P450-4 , and P450-2 in the cluster increased in the G-group strain and not in the F-group strain. We hypothesized that complementation by GA genes in the G-group strain would be required to increase GA production in the F-group strain. However, we found that this occurred with a single gene complementation of DES , P450-1 , P450-4 , or P450-2 . Simultaneous increase in the expression of P450-1 , P450-4 , and P450-2 were detected in the complementary transformants. Moreover, the same phenomenon was observed by reintegration of its own P450-1 . Our results suggest the presence of unknown regulatory mechanisms of the GA gene cluster in F . fujikuroi.
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Link to article at publishers website
Database assignments for author(s): Iori Imazaki, Shin-ichi Fuji, Masafumi Shimizu, Koji Kageyama

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium fujikuroi