Plant Pathology (2019) 68, 42-48

M.M. Mohd. Nadzir, F.M. Vieira Lelis, B. Thapa, A. Ali, R.G.F. Visser, A.W. van Heusden and J.M. van der Wolf (2019)
Development of an in vitro protocol to screen Clavibacter michiganensis subsp. michiganensis pathogenicity in different Solanum species
Plant Pathology 68 (1), 42-48
Abstract: Clavibacter michiganensis subsp. michiganensis (Cmm) is a quarantine organism in Europe and in many other countries. It is one of the most severe bacterial pathogens affecting tomato. Screening tomato plants for their resistance level to Cmm requires a large amount of space under quarantine conditions and is therefore costly. This project developed a new inoculation protocol on in vitro tomato plants to facilitate a more economic and higher throughput disease screening. A new method using the PathoScreen system was tested to localize green fluorescent protein-tagged Cmm in planta and to quantify the pathogen based on the percentage of corrected GFP (cGFP%). The system was sensitive in detecting the GFP-tagged Cmm in the shoots, but in the roots a high autofluorescence masked detection and thus sensitivity of the assay. The in vitro protocol was tested on several wild relatives of tomato, which were previously screened in a greenhouse assay. The correlation between wilt symptoms in vitro and wilt symptoms in the greenhouse was overall moderate (r = 0.6462). The protocol worked well in differentiating the two parents that were used in the mapping studies. This study shows that the in vitro protocol can be efficiently used for resistance breeding in many tomato genotypes.
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Database assignments for author(s): Richard G.F. Visser, Adriaan W. van Heusden, Jan M. van der Wolf

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Clavibacter michiganensis	Tomato (Solanum lycopersicum)