Plant Pathology (2014) 63, 447-455

R. Lahlali and G. Peng (2014)
Suppression of clubroot by Clonostachys rosea via antibiosis and induced host resistance
Plant Pathology 63 (2), 447-455
Abstract: The mechanism of the biofungicide Prestop^® (Clonostachys rosea) was investigated for control of clubroot (Plasmodiophora brassicae) on canola. The key product components were partitioned and assessed for their effect on pathogen resting spores, root hair infection (RHI) and disease development using light microscopy, quantitative PCR and different application treatments during infection. The whole product of Prestop was consistently more effective than the C. rosea conidial suspension or product filtrate alone in reducing RHI and clubroot development. This biofungicide showed little effect on germination or viability of resting spores. Two-application treatments at seeding and 7-14 days after seeding achieved greater clubroot control than a single application of the biofungicide at either seeding or post-seeding stage. This may indicate the need to maintain a high biofungicide dose in the soil during primary and secondary infection. This biocontrol fungus colonized the rhizosphere and interior of canola roots extensively, and possibly induced plant resistance based on up-regulation of the genes that are involved in jasmonic acid (BnOPR2), ethylene (BnACO) and phenylpropanoid (BnOPCL, BnCCR) biosynthetic pathways. It is concluded that the biofungicide Prestop suppressed clubroot on canola at least via root colonization and induced systemic resistance (ISR), and the latter may be through the modulation of phenylpropanoid and jasmonic acid/ethylene metabolic pathways elicited by the fungus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Gary Peng, Rachid Lahlali

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
resistance/tolerance/defence of host
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plasmodiophora brassicae	Rape/canola (Brassica napus)
Clonostachys rosea (antagonist)	Plasmodiophora brassicae	Rape/canola (Brassica napus)