Plant Pathology (2001) 50, 124-129

M. Nicolaisen, H.N. Rasmussen, K. Husted and S.L. Nielsen (2001)
Reverse transcription–detection of immobilized, amplified product in a one-phase system (RT–DIAPOPS) for the detection of Potato virus Y
Plant Pathology 50 (1), 124-129
Abstract: An RT–DIAPOPS (reverse transcription–detection of immobilized, amplified product in a one-phase system) assay was developed for the detection of potato virus Y (PVY). The sensitivity of the assay was 0·5 pg when virions were added directly to the RT reaction. The assay was capable of detecting all isolates investigated, representing several taxonomic groups of PVY. The RT–DIAPOPS offers an attractive alternative to RT–polymerase chain reaction (PCR) as it does not depend on gel electrophoresis to detect virus-specific PCR products. Finally, as amplicons are covalently attached to a solid phase, it minimizes the risk of contamination.
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Database assignments for author(s): Mogens Nicolaisen, Steen Lykke Nielsen

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y