Plant Pathology (2000) 49, 101-107

Y.H. Hsu, P. Annamalai, C.S. Lin, Y.Y. Chen, W.C. Chang and N.S. Lin (2000)
A sensitive method for detecting Bamboo mosaic virus (BaMV) and establishment of BaMV-free meristem-tip cultures
Plant Pathology 49 (1), 101-107
Abstract: A sensitive method was used to detect bamboo mosaic virus (BaMV) and its associated satellite RNA (satBaMV) by ³²P- and digoxigenin (Dig)-labelled probes synthesized from cDNA clones of BaMV genomic (L probe) and satBaMV (S probe) RNA. Both the ³²P- and Dig-labelled L and S probes could detect as little as 490 pg of BaMV viral RNA by slot- and dot-blot hybridization. In infected leaf extracts, ³²P-labelled L and S probes detected virus at 25-fold higher dilutions than Dig-labelled probes, which were also successfully used to detect BaMV infection in plants derived from meristem-tip culture. However, immunoassays failed to detect BaMV in meristem culture. By dot-blot hybridization assays, 25% of the seedlings were shown to be virus-free. These results suggest that a highly sensitive method for the detection of BaMV infection is required for the establishment of BaMV-free cultures. Meristem-tip culture also provides an efficient method for obtaining virus-free bamboo plants.
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Research topic(s) for pests/diseases/weeds:
identification/taxonomy
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bamboo mosaic virus		Taiwan