Plant Disease (2003) 87, 679-685

R.P. Singh, D.L. McLaren, X. Nie and M. Singh (2003)
Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection
Plant Disease 87 (6), 679-685
Abstract: Surveys of commercial and seed potato fields for virus diseases (1998 to 2002) in Manitoba established that Potato virus Y (PVY) is of concern in seed potato production. To determine the prevalence of PVY strains, PVY-infected tubers identified by reverse transcription-polymerase chain reaction (RT-PCR) from surveys (2000 to 2001) were grown for symptom expression and strain characterization by strain-specific RT-PCR, bioassays, and serological assays. Of the samples collected (2000 to 2001) and tested by RT-PCR, 4.0% contained PVY. Further analysis of the PVY-positive samples by a duplex RT-PCR facilitating the simultaneous detection of common (PVY^O) and tobacco veinal necrosis strains (PVY^N/NTN) indicated that 37.5% contained PVY^O and 63.5% contained PVY^N-type isolates. Analysis of the PVY^N-type samples using three monoclonal antibodies (MAbs) showed that all reacted with only the PVY^O MAbs and not with the PVY^N-specific MAb. Partial nucleotide sequences of both ends of PVY-RNA showed that the PVY^N-type isolates resembled those reported in 1996 from Manitoba. These isolates are designated as PVY^N:O. In view of the increased incidence of PVY^N:O in one production area, seed tubers imported from other provinces of Canada and the neighboring United States were analyzed for PVY^N:O. The PVY^N:O was detected in imported seeds from Minnesota, Montana, and North Dakota.
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Database assignments for author(s): Xianzhou Nie, Rudra P. Singh

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y		U.S.A. (mid N)
	Potato virus Y		Canada (west)
	Potato virus Y	Potato (Solanum tuberosum)	U.S.A. (NW)