Plant Disease (2001) 85, 131-136

V. Toussaint, C.E. Morris and O. Carisse (2001)
A new semi-selective medium for Xanthomonas campestris pv. vitians, the causal agent of bacterial leaf spot of lettuce
Plant Disease 85 (2), 131-136
Abstract: A semi-selective medium containing maltose, methyl green, and antibiotics (MMG) was developed for the isolation of Xanthomonas campestris pv. vitians. The semi-selective medium was evaluated based on plating efficiency of X. campestris pv. vitians in cell suspensions of pure cultures from leaves and soil. MMG medium allowed recovery of 5.7 to 30.6% of the X. campestris pv. vitians colonies recovered on nutrient agar and 0.1 to 8.4% of those recovered on 1/10-strength tryptic soy agar. MMG inhibited growth of most background bacteria and allowed reliable identification of X. campestris pv. vitians. The semi-selective medium contained (per liter) maltose (10 g), tryptone (5 g), K₂HPO₄ (3.5 g), KH₂PO₄ 2.75 g), trace elements (0.02 to 1.0 mg), methyl green (2 ml of a 1% aqueous solution), amoxicillin (32 mg), cephalothin (32 mg), cycloheximide (50 mg), and agar (15 g).
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Database assignments for author(s): Cindy E. Morris, Odile Carisse, Vicky Toussaint

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas campestris pv. vitians	Lettuce (Lactuca)	France
	Xanthomonas campestris pv. vitians	Lettuce (Lactuca)	Greece
	Xanthomonas campestris pv. vitians	Lettuce (Lactuca)	Canada (east)
	Xanthomonas campestris pv. vitians	Lettuce (Lactuca)	U.S.A. (SE)
	Xanthomonas axonopodis