Phytopathology (2016) 106, 710-718

Sharat Kumar Pradhan, Deepak Kumar Nayak, Elssa Pandit, Lambodar Behera, Annamalai Anandan, Arup Kumar Mukherjee, Srikanta Lenka and Durga Prasad Barik (2016)
Incorporation of bacterial blight resistance genes into lowland rice cultivar through marker-assisted backcross breeding
Phytopathology 106 (7), 710-718
Abstract: Bacterial blight (BB) of rice caused by Xanthomonas oryzae pv. oryzae is a major disease of rice in many rice growing countries. Pyramided lines carrying two BB resistance gene combinations (Xa21+xa13 and Xa21+xa5) were developed in a lowland cultivar Jalmagna background through backcross breeding by integrating molecular markers. In each backcross generation, markers closely linked to the disease resistance genes were used to select plants possessing the target genes. Background selection was continued in those plants carrying resistant genes until BC₃ generation. Plants having the maximum contribution from the recurrent parent genome were selected in each generation and hybridized with the recipient parent. The BB-pyramided line having the maximum recipient parent genome recovery of 95% was selected among BC₃F₁ plants and selfed to isolate homozygous BC₃F₂ plants with different combinations of BB resistance genes. Twenty pyramided lines with two resistance gene combinations exhibited high levels of tolerance against the BB pathogen. In order to confirm the resistance, the pyramided lines were inoculated with different X. oryzae pv. oryzae strains of Odisha for bioassay. The genotypes with combination of two BB resistance genes conferred high levels of resistance to the predominant X. oryzae pv. oryzae isolates prevalent in the region. The pyramided lines showed similarity with the recipient parent with respect to major agro-morphologic traits.
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Link to article at publishers website
Database assignments for author(s): Arup Mukherjee

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas oryzae	Rice (Oryza)