Phytopathology (2015) 105, 550-554

Keiichiro Matsukura, Tomomi Towata, Kazuhiro Yoshida, Junichi Sakai, Mitsuru Okuda, Masatoshi Onuki and Masaya Matsumura (2015)
Quantitative analysis of Southern rice black-streaked dwarf virus in Sogatella furcifera and virus threshold for transmission
Phytopathology 105 (4), 550-554
Abstract: We investigated Southern rice black-streaked dwarf virus (SRBSDV) accumulation in a vector insect, the whitebacked planthopper (Sogatella furcifera), to elucidate the association of virus accumulation in the vector with virus transmission efficiency. Real-time quantitative reverse transcription polymerase chain reaction analysis confirmed that this virus is transmitted in a persistent-propagative manner. SRBSDV was successfully transmitted by S. furcifera males in which RNA accumulation of the capsid protein gene of SRBSDV was >10³ in the whole body of S. furcifera, indicating that the threshold accumulation of the virus RNA for virus transmission is 10³ in an S. furcifera male. The SRBSDV detection rate in the immigrant population of S. furcifera was high in 2011 (39.5%); however, most of the insects contained fewer than 10³ RNAs of the capsid protein gene. This result indicates that the risk of SRBSDV epidemics could be estimated from the proportion of virus-transmissible S. furcifera (i.e., S. furcifera that contained more than 10³ RNAs of the virus capsid protein gene) rather than the SRBSDV detection rate in S. furcifera.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Masaya Matsumura, Mitsuru Okuda

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sogatella furcifera
	Southern rice black-streaked dwarf virus