Phytopathology (2002) 92, 1167-1174

M.G. Redinbaugh, D.L. Seifers, T. Meulia, J.J. Abt, R.J. Anderson, W.E. Styer, J. Ackerman, R. Salomon, W. Houghton, R. Creamer, D.T. Gordon and S.A. Hogenhout (2002)
Maize fine streak virus, a new leafhopper-transmitted rhabdovirus
Phytopathology 92 (11), 1167-1174
Abstract: A previously uncharacterized virus was isolated from fall-planted sweet corn (Zea mays L., Syngenta GSS 0966) leaves showing fine chlorotic streaks. Symptomatic plants were negative in enzyme-linked immunosorbent assay against many maize viruses, but reacted weakly with antisera to Sorghum stunt mosaic virus suggesting a distant relationship between the viruses. The virus was readily transmitted by vascular puncture inoculation (VPI), but not by leaf-rub inoculation. Symptoms on maize included dwarfing and fine chlorotic streaks along intermediate and small veins that developed 12 to 17 days post-VPI. The isolated virus was bacilliform (231 ± 5 nm long and 71 ± 2 nm wide), with a knobby surface, and obvious helical structure typical of rhabdovirus morphology. Nucleorhabdovirus virions were observed by transmission electron microscopy of infected maize leaf tissue sections. Proteins unique to infected plants were observed in extracts of infected leaves, and the isolated virion contained three proteins with molecular masses 82 ± 2, 50 ± 3, and 32 ± 2 kDa. Preliminary sequence analysis indicated the virus had similarity to members of the family Rhabdoviridae. The virus was transmitted by Graminella nigrifrons under persistent conditions. The data indicate the virus, provisionally designated Maize fine streak virus, is a new species in the genus Nucleorhabdovirus.
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(original language: English)
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Database assignments for author(s): Saskia A. Hogenhout, Rebecca Creamer, Margaret G. Redinbaugh

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Graminella nigrifrons	Maize/corn (Zea mays)	U.S.A. (SE)
	Gammanucleorhabdovirus maydis	Maize/corn (Zea mays)	U.S.A. (SE)