Phytopathologia Mediterranea (2010) 49, 80-83

Chiaraluce Moretti and Roberto Buonaurio (2010)
Immature walnut fruit inoculation for evaluation of Brenneria nigrifluens pathogenicity
Phytopathologia Mediterranea 49 (1), 80-83
Abstract: A reliable, reproducible, rapid and specific test for the pathogenicity of Brenneria nigrifluens, the causal agent of shallow bark canker of Persian walnut, was developed. When the mesocarp of immature walnut fruits was infiltrated by syringe with bacterial suspensions (10⁸ cells mL^-1) of B. nigrifluens, necrosis and reddish brown exudates started to appear at the inoculation sites, as early as 2 days after inoculation. No symptoms were detected in control fruits or in fruits inoculated with other bacteria frequently associated with walnut cankers. This pathogenicity test saves time and space when compared with the plant stem inoculation technique, in which the canker symptoms do not appear until at least 1 month after inoculation.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
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Database assignments for author(s): Chiaraluce Moretti, Roberto Buonaurio

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Brenneria nigrifluens	Walnut/butternut (Juglans)