Phytopathologia Mediterranea (2004) 43, 263-267

Francesca De Luca, Aurelio Reyes, Augusta Agostinelli, Carla De Giorgi and Franco Lamberti (2004)
Molecular diagnostics and variability of longidorid nematodes
Phytopathologia Mediterranea 43 (2), 263-267
Abstract: PCR-RFLP and sequencing approaches of ribosomal DNA are being used to study taxonomy, molecular identification and phylogeny of plant parasitic nematodes. In this paper, we discuss on the usefulness of ITS PCRRFLP analysis to differentiate among longidorid species. In addition, we examined how well ITS PCR-RFLP differentiated between longidorid species, and how well sequencing of two different ribosomal regions, the ITS containing region and D1-D2 domains of the 26S rDNA, were able to infer phylogenetic relationships among those same species. These methods and their advantages in identifying longidorids and establishing their phylogenetic relationships are examined and discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Franco Lamberti, Francesca De Luca

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Longidorus (genus)
	Xiphinema (genus)