Phytopathologia Mediterranea (2004) 43, 177-186

Faten Gorsane, Imene Fekih-Hassen, Mark K. Nakhla, Hatem Fakhfakh, Douglas P. Maxwell and Mohamed Marrakchi (2004)
Molecular evidence of Tomato yellow leaf curl virus-Sicily spreading on tomato, pepper and bean in Tunisia
Phytopathologia Mediterranea 43 (2), 177-186
Abstract: Unusual symptoms including yellowing, stunting, curling, crumpling and plant size reduction were observed in tomato fields and green houses in Tunisia. These symptoms, generally associated with the Tomato yellow leaf curl virus (TYLCV) complex, have become increasingly common in recent years. In order to ascertain the molecular characteristics of Tunisian isolates by PCR, both the coat protein gene and the intergenic region of eleven isolates were amplified using specific primers, and sequenced. The PCR procedure also allowed the amplification of viral DNA fragments using a bean total DNA as a template. Phylogenetic analysis suggested that these Tunisian isolates clustered with a Sicilian isolate of TYLCSV-Sic. This is the first report of the involvement of this viral species in Phaseolus vulgaris disease.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato yellow leaf curl virus	Green pepper/chilli (Capsicum)	Tunisia
	Tomato yellow leaf curl virus	Tomato (Solanum lycopersicum)	Tunisia
	Tomato yellow leaf curl virus	Beans (Phaseolus)	Tunisia