Phytoparasitica (2002) 30 (3) - Heterokaryon compatibility in ...

Nadia Korolev (2002)
Heterokaryon compatibility in Verticillium dahliae tested by comparing nit and auxotrophic mutants
Phytoparasitica 30 (3)
The 23rd Congress of the Israeli Phytopathological Society - February 11-12, 2002
Abstract: Isolates of Verticillium dahliae defined as VCG1, VCG2A, VCG2B and VCG4B using nitrate nonutilizing (nit) mutants were marked by mono- or diauxotrophic mutations and by resistance to carbendazim. Heterokaryon compatibility between marked strains belonging to the same or to different VCGs was tested using the 'hanging drops' or the 'blocks' technique. Combinations involving marked strains from the same VCG were successful (23 combinations of different auxotrophic markers), whereas most inter-VCG pairings failed (48 combinations of auxotrophs). In a few cases prototrophic growth formed by auxotrophs from different VCGs was proved to be cross-feeding using interposed cellophane membrane. Reversion to prototrophy in one parental strain was the reason for prototrophic growth in a few other cases. In some inter-VCG combinations, very slow or inconspicuous prototrophic growth was observed, which is similar to weak complementation occasionally observed at the contact zone between complementary nit mutants belonging to different VCGs. It was concluded that VCGs defined by nit and by auxotrophic mutants coincide. Parasexual cycle, tested by recovering prototrophic recombinants and diploid conidia from high-temperature-treated heterokaryons, was demonstrated between isolates belonging to the same VCG.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Nadia Korolev

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Verticillium dahliae