Phytoparasitica (2002) 30 (3) - Effect of pH on Colletotrichum ...

N. Drori, H. Kramer-Haimovich, A. Dinoor, Y. Okon, O. Pines and D. Prusky (2002)
Effect of pH on Colletotrichum gloeosporioides PacC and pelB expression and its relation to pectate lyase secretion by the pathogen
Phytoparasitica 30 (3)
The 23rd Congress of the Israeli Phytopathological Society - February 11-12, 2002
Abstract: Accumulation of ammonia during Colletotrichum gloeosporioides attack and its effect on pH alkalization is considered as a virulence factor for fungal attack on fruits. The fungus-induced increase in pH to 6.0, changes the pattern of secretion. Secretion of pectate lyase was detected at an initial pH not lower than 5.4 and increased up to pH 6.0. At pH 4.0, neither expression nor secretion was observed. Also nitrogen source was found to affect secretion, and this was observed also at the transcription level. Contrary to pelB, transcriptional activation of pg1, encoding the endopolygalacturonase, accumulated similarly at pH 4.0 and 6.0. Analysis of the promotor region of pelB showed that it contains seven PacC consensus-binding sites. In order to determine whether pelB is pH-regulated by a signal transduction process, we cloned a putative transcription factor-encoding gene, pac1. Its transcription accumulates parallel to ambient pH. Our results suggest that ambient pH is a regulatory factor for processes linked to virulence and that these processes may be under the molecular regulation of a conserved pH-dependent signaling pathway.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Dov Prusky

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Colletotrichum gloeosporioides