Phytoparasitica (2002) 30, 493-501

C. Varveri, N. Vassilakos and F. Bem (2002)
Characterization and detection of Cucumber green mottle mosaic virus in Greece
Phytoparasitica 30 (5), 493-501
Abstract: The host range specificity of Greek isolates of Cucumber green mottle mosaic virus (CGMMV) was determined and the ability of the virus to retain its infectivity in naturally contaminated soil, after storage at 4°C for at least 10 months, was established. An immunocapture RT-PCR protocol was developed for the detection of the virus and proved to be 10⁵ times more sensitive than DAS-ELISA and 10² times more sensitive than F(ab')₂ -ELISA using F(ab')₂ fragments that were prepared from an antiserum, which was raised against a local virus isolate. In order to clarify dissimilarities that were revealed between the coat protein (CP) genes of the Greek isolates after restriction mapping of their respective PCR products, the CP genes of three isolates were sequenced and found to be very similar but not identical to the CP gene of CGMMV-SH.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Christina Varveri

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
population dynamics/ epidemiology

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber green mottle mosaic virus		Greece