Phytoparasitica (1998) 26 (1) - RNA transcripts of banana ...

P.R. Beetham, G.J. Hafner, R.M. Harding and J.L. Dale (1998)
RNA transcripts of Banana bunchy top virus (BBTV)
Phytoparasitica 26 (1)
Xth International Congress of Virology, August 11-16, 1996, Binyanei haOoma, Jerusalem, Israel, lecture
Abstract: We have mapped the RNA transcripts of BBTV components 1-6. Northern hybridization studies and 3' RACE have identified seven polyadenylated RNAs associated with the BBTV genome. Interestingly, component 1, which contains one major open-reading frame (ORF), a putative replicase-associated protein (RAP), has been shown to transcribe a mRNA from an ORF internal to the major RAP ORF. This transcript encodes a putative M, 5K protein of unknown function. It is possible this internal ORF may be involved with regulation of viral replication. Both component 1 RNA transcripts have been identified in tobacco plants transformed with a vector containing the major ORF of component 1. Component 2 was found to have an RNA transcript which mapped to a small ORF. This is the first report of an ORF encoding a putative protein from component 2. The RNA transcripts associated with components 3-6 are all from previously reported ORFs. We have mapped the important 3' untranslated regions, including the conserved termination signals. The poly(A) signal, poly(A) site, GT-rich region and an upstream element (A/C/TTGTAA) seem to be also conserved in other ssDNA viruses.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Robert M. Harding

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Banana bunchy top virus