Phytoparasitica (1998) 26, 199-212

J.A. Navas-Cortés, E. Pérez-Artés, R.M. Jiménez-Díaz, A. Llobell, B.W. Bainbridge and J.B. Heale (1998)
Mating type, pathotype and RAPDs analysis in Didymella rabiei, the agent of ascochyta blight of chickpea
Phytoparasitica 26 (3), 199-212
Abstract: Eleven pathotype groups (A-K), including five not previously reported, of Didymella rabiei (anamorph Ascochyta rabiei), representing isolates of the pathogen from Ascochyta blightaffected chickpeas mainly from India, Pakistan, Spain and the USA, were characterized using 44 single-spore isolates tested against seven differential chickpea lines. Of 48 isolates tested for mating type, 58% belonged to MAT 1-1 and 42% to MAT 1-2. Thirty-nine D. rabiei isolates, as well as two isolates of Ascochyta pisi and six isolates of unrelated fungi, were analyzed using Randomly Amplified Polymorphic DNAs (RAPDs) employing five primers (P2 at 40°C, and OPA3, OPC1, OPC11 and OPC20 at 35°C). Computer cluster analysis (UPGMA / NTSYS-PC) detected a relatively low level of polymorphism among all the D. rabiei isolates, although at ca 7% dissimilarity, ca 10 RAPD groups [I-X] were demarcated, as well as subclustering within the larger groups. By the same criteria, the maximum dissimilarity for the whole population of D. rabiei isolates was ca 13%. No correlation was found between different RAPD groups, pathotype, or mating type of D. rabiei, although some evidence of clustering based on geographic origin was detected. The use of RAPDs enabled us to identify specific DNA fragments that may have a potential use as genetic markers in sexual crosses, but none which could be used as virulence markers.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Juan A. Navas-Cortes, Rafael M. Jimenez-Diaz, Encarnación Pérez-Artés

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ascochyta rabiei		France
	Ascochyta rabiei		Greece
	Ascochyta rabiei		Italy
	Ascochyta rabiei		Turkey
	Ascochyta rabiei		India
	Ascochyta rabiei		Pakistan
	Ascochyta rabiei		Morocco
	Ascochyta rabiei	Chickpea (Cicer arietinum)	Spain (continental)