Phytoparasitica (1997) 25, p. 75 (Antignus et al.)

Y. Antignus, O. Lachman, M. Pearlsman and S. Cohen (1997)
Mutational analysis of virion sense genes of Tomato yellow leaf curl virus (TYLCV)
Phytoparasitica 25 (1), 75-75
The Xth International Congress of Virology, August 11-16, 1996, Jerusalem, Israel, lecture
Abstract: Deletion, frameshift, and single amino acid mutations were inserted into open reading frames (ORFs) V1 and V2 (capsid protein) of TYLCV. The ability of these mutants to replicate, to spread and to induce symptoms was tested both in leaf discs and in intact plants. A deletion mutant that lacked the carboxy half of the coat protein (CP) gene and a frameshift mutant, in which an early termination resulted in removal of 13 amino acids from the carboxy terminus of the virus CP and changing of an additional nine amino acids, were found non-infectious. Two other double mutants, in which a single amino acid was changed in the overlapping part of V1 and V2, and one mutant in which a deletion was introduced exclusively to V1, were able to spread systemically, but infections remained symptomless; the synthesis of ssDNA was significantly lower than in an unmutated clone. The latter three mutants were acquired and transmitted by Bemisia tabaci.

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato yellow leaf curl virus