Phytoparasitica (1996) 24, 158-159
Smadar Lischinsky, S. Droby, Shulamit Manulis, Lea Cohen and Batia Horev (1996)
Evaluation of biocontrol activity of epiphytic yeasts of citrus fruit against green mold decay caused by Penicillium digitatum
Phytoparasitica 24 (2), 158-159
The 17th Congress of the Israeli Phytopathological Society, February 19-20, 1996 (Bet Dagan, Israel), lecture
Abstract: This study was aimed at isolation and characterization of the epiphytic yeast population of citrus fruit antagonistic to green mold decay caused by Penicillium digitatum. Ability to grow on elevated concentrations of NaCl, glucose and a wide range of temperatures was used as the criterion for the initial selection. Of 202 yeast isolates, selected at random following plating of surfaces, washings and isolations from wounds, 64 grew at temperatures ranging from 5 to 43°C. Of 64 isolates, approximately 60 were found to grow on potato dextrose agar amended with either 10% NaCl or 50% glucose. Following an assay performed on wounded grapefruit to test biocontrol activity, 46 isolates exhibited 70% or more inhibition of infection. Among these antagonists, four yeast isolates were highly effective against P. digitatum on grapefruit. Complete inhibition of infection of surface wounds resulted when those yeast isolates were applied at a concentration of 108 cells/ml. RAPDPCR analysis showed that the population of the 64 yeasts tested consisted mainly of three groups of isolates, suggesting the presence of only three different yeast species. Classical identification (based on ability to grow on various carbon sources) of representatives of the three groups showed only two yeast species: Pichia guilliermondii and Debaryomyces hansenii.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
application technology
evaluation - screening - selection
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Penicillium digitatum | Stored fruits/vegetables | |||
| Debaryomyces hansenii (antagonist) | Penicillium digitatum | Stored fruits/vegetables | ||
| Meyerozyma guilliermondii (antagonist) | Penicillium digitatum | Stored fruits/vegetables |
