Pesticide Science (1995) 44, 33-38

Timothy Joseph-Horne, Angelina Carelli, Derek Hollomon and Steven L. Kelly (1995)
Investigation of azole resistance in the Ustilago maydis sterol demethylase mutant erg 40
Pesticide Science 44 (1), 33-38
Abstract: Resistance to azole fungicides in Ustilago maydis (DC) Corda has been examined using the mutant erg 40, a newly isolated mutant Tri^R-1 and erg 40 revertants. Azole-induced growth arrest of the wild type did not support an obvious role for 3,6-diol in the mode of action has is clear for Saccharomyces cerevisiae Meyer ex Hansen. The level of microsomal P450 of erg 40 was identical to that of the parent, and reversion analysis showed no evidence of mutation in the sterol Delta⁵⁽⁶⁾ desaturase, as would be expected for a S. cerevisiae mutant accumulating 14alpha-methylfecosterol. Resistance appeared to be due to a single mutation in P450 14alpha dm. It is proposed that the orthologous forms of fungal sterol Delta⁵⁽⁶⁾ desaturases have varied responses when attempting to utilise 14alpha-methylated substrates.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Derek W. Hollomon

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Mycosarcoma maydis