Pest Management Science (2020) 76, 2932-2943

Tian-Xing Jing, Di-Fei Wang, Yun-Peng Ma, Li-Li Zeng, Li-Wei Meng, Qiang Zhang, Wei Dou and Jin-Jun Wang (2020)
Genome-wide and expression-profiling analyses of the cytochrome P450 genes in Bactrocera dorsalis (Hendel) and screening of candidate P450 genes associated with malathion resistance
Pest Management Science 76 (9), 2932-2943
Abstract:
BACKGROUND
Bactrocera dorsalis (Hendel) is a notorious agricultural pest worldwide, and its resistance to insecticides is a major obstacle in successful control. Cytochrome P450s (P450s) are major metabolic enzymes associated with insecticide resistance. The genome of B. dorsalis was sequenced recently, allowing an integrated genome-wide analysis of P450 genes (P450s) and the analysis of correlations between these genes and insecticide resistance in this pest.
RESULTS
Totally, 101 P450s were identified in the B. dorsalis genome and classified into four clans, 25 families and 57 subfamilies. Quantitative reverse transcription polymerase chain reaction results showed that most of these genes were highly expressed in adults (46) and in metabolic tissues, including the fatbody (63), midgut (61) and Malphagian tubules (66). In a malathion-resistant strain, 13 and 9 genes were significantly upregulated and downregulated, respectively, compared with a susceptible strain, and these genes were screened as candidate genes associated with malathion resistance.
CONCLUSION
This study provides useful information for understanding the evolution and potential functions of P450s in B. dorsalis, and the results lay the foundation for further studies on the correlations between P450s and malathion resistance in B. dorsalis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jin-Jun Wang

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Bactrocera dorsalis