Pest Management Science (2019) 75, 2802-2809

Kaiyang Feng, Yuwei Yang, Xiang Wen, Shiyuan Ou, Ping Zhang, Qian Yu, Yichao Zhang, Guangmao Shen, Zhifeng Xu, Jinhang Li and Lin He (2019)
Stability of cyflumetofen resistance in Tetranychus cinnabarinus and its correlation with glutathione-S-transferase gene expression
Pest Management Science 75 (10), 2802-2809
Abstract:
BACKGROUND
Cyflumetofen is an outstanding acaricide with a novel mode of action. Tetranychus cinnabarinus, an important agricultural pest, is notorious for developing resistance to most classes of acaricides rapidly and results in enormous loss for the economy. Our previous study had pointed out glutathione S-transferase (GSTs) significantly contributed to the cyflumetofen-resistance formation in T. cinnabarinus, but the more specific mechanism needed to be further investigated.
RESULTS
The unstable resistance was observed in cyflumetofen-resistant strain (CyR)under acaricide-free condition. The activity of GSTs increased along with the development of resistance. The expressions of 13 GST genes were detected in CyR and susceptible strain (SS), of which six genes were overexpressed in CyR and the TcGSTm02 was selected as the representative for functional study. The expression of TcGSTm02 changed along with the resistant level of CyR with the same trend. Recombinant protein of TcGSTm02 with high activity was successfully obtained by E. coli expression system, whose activity could be inhibited by cyflumetofen (IC₅₀ = 0.23 mM). Recombinant TcGSTm02 could effectively decompose cyflumetofen, and catalyze GS^- to conjugate with cyflumetofen.
CONCLUSION
All clues confirmed that GSTs strongly associated with cyflumetofen-resistance and a representative gene, TcGSTm02, showed function on contributing the evolution of cyflumetofen-resistance in T. cinnabarinus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Lin He

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tetranychus cinnabarinus