Pest Management Science (2016) 72, 1433-1439

Jun Hu and Yu-Xian Xia (2016)
F₁-ATP synthase α-subunit: a potential target for RNAi-mediated pest management of Locusta migratoria manilensis
Pest Management Science 72 (7), 1433-1439
Abstract:
BACKGROUND
The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase (F₀F₁-ATPase) uses proton or sodium motive force to produce 90% of the cellular ATP, and the α-subunit of F₁-ATP synthase (ATP5A) is vital for F₁-ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi-mediated pest management of L. migratoria.
RESULTS
Lm-ATP5A was cloned and characterised. Lm-ATP5A is expressed in all tissues. Injection of 100 ng of the double-stranded RNA of ATP5A (dsATP5A) knocked down the transcription of the target gene and caused mortality in 1.5–5 days. The Lm-ATP5A protein level, the oligomycin-sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection.
CONCLUSION
These findings demonstrated the essential roles of Lm-ATP5A in L. migratoria and identified it as a potential target for insect pest control.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Yuxian Xia

Research topic(s) for pests/diseases/weeds:
control - general
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Locusta migratoria