Pest Management Science (2013) 69, 652-660

B. van Kretschmar, Ana R. Cabrera, Julius R. Bradley and R. Michael Roe (2013)
Novel adult feeding disruption test (FDT) to detect insecticide resistance of lepidopteran pests in cotton
Pest Management Science 69 (5), 652-660
Abstract:
BACKGROUND
Resistance monitoring is an important aspect of insect resistance management and the preservation of insecticide efficacy. The adult vial test (AVT) is most often used for resistance monitoring for a variety of insects. A potential alternative method is feeding disruption where resistant insects are distinguished from susceptible insects on the basis of their ability to feed on insecticide in nectar containing a colorimetric marker to measure feeding. The advantages of a feeding disruption test (FDT) for lepidopteran adults might include a more rapid assay than AVT, an assay format easier to prepare, a bioassay applicable to both oral and contact insecticides and the provision of food and water during the course of the test. The objective of the present work was to determine the feasibility of an adult FDT.
RESULTS
Heliothis virescens moths fed permethrin and spinosad in dyed nectar yielded dose-dependent ingestion, fecal production and mortality data. A permethrin diagnostic dose distinguished pyrethroid-resistant from pyrethroid-susceptible moths, based on fecal production.
CONCLUSION
Proof of concept was demonstrated for an adult FDT in which resistant moths were distinguished from susceptible moths on the basis of the ability of the insect to feed on insecticide in dyed nectar and produce dyed feces.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): R. Michael Roe

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Heliothis virescens