Neotropical Entomology (2004) 33, 179-187

Daniel R. Sosa-Gomez, Katiaíres E. Delpin, Alvaro M.R. Almeida and Edson Hirose (2004)
Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis
Neotropical Entomology 33 (2), 179-187
Abstract: The Neotropical Brown Stink Bug (NBSB), Euschistus heros (Fabricius), has a wide distribution in Brazilian soybean fields, being more important in the central region of the country. The species is the main target of insecticide applications for stink bug pest control. We determined the. variability among and within populations of the NBSB by random amplification of polymorphic DNA (RAPD) analysis. Samples of NBSB were collected in soybean fields from Ubiratã (PR); Londrina (PR), Centenário do Sul (PR), Cândido Mota (SP), Ponta Porã (MS) and Sapezal (MT). Genomic DNA was extracted from the head to minimize DNA contamination by endoparasites. DNA was amplified with 10 mer primers. Fifteen primers produced 246 bands. The genetic similarity matrix was obtained, based on RAPD allele frequencies, using Nei's 1972 genetic distance. NSBS collected from the same geographical region clustered together. The populations from Londrina and Centenário do Sul were genetically closer than the others and stink bugs collected in Candido Mota were closer to Ponta Pora population. The Sapezal geographical population was the most divergent from the others. Females and males clustered separately inside each geographical population, implying that RAPD permits gender discrimination. We did not observe individuals from one region clustering together with stink bugs from another region. The number of polymorphic loci from the different populations ranged between 40.6% and 52.1%. The gene flow indexes (overall Nm = 0.8307) were lower than that observed for Anticarsia gemmatalis Hübner and Helicoverpa armigera (Hübner) suggesting that in stink bug populations gene flow is lower than in the noctuid moths.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Daniel R. Sosa-Gomez

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
surveys/sampling/distribution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Euschistus heros	Soybean (Glycine max)	Brazil (south)
	Euschistus heros	Soybean (Glycine max)	Brazil (NW)