Nematropica (2001) 31, 283-290

S. Supramana, S.A. Lewis, J.D. Mueller, B.A. Fortnum and R.E. Ballard (2001)
Biology of Columbia lance nematode (Hoplolaimus columbus Sher) on soybean excised root culture
Nematropica 31 (2), 283-290
Abstract: Feeding, egg development and oviposition, embryology, and postembryonic development of Hoplolaimus columbus were observed on soybean (cultivar Hutcheson) excised root culture in Gamborg's B5 medium (pH 5.7, 1.3% agar). Petri plate sterile cultures were placed inverted on a heated microscope stage (30° C) and observations were conducted using a color video camera and a time lapse digital videocassette recorder. Oöcytes were observed in each gonad 38 hours after feeding commenced and an egg possessing a terminal stalk was laid 96 hours later. Eggs were deposited in the one-celled stage and a total of 217 hours (9 days) was required for a newly laid egg to develop into a hatching second-stage juvenile at 30° C. Postembryonic development consisted of three juvenile stages and an adult female with prior feeding occurring before molts and oviposition. The nematode fed ectoparasitically on the subepidermal cells and endoparasitically in the cortex and outer vascular tissue and remained at the infection site for 2-9 days.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Hoplolaimus columbus	Soybean (Glycine max)