Nematology (2000) 2, p. 770 (Lecouls et al.)

Anne-Claire Lecouls, Maria-Jose Rubio-Cabetas, Elisabeth Dirlewanger, Henri Duval, Georges Salesses and Daniel Esmenjaud (2000)
Marker-assisted selection (MAS) of the Ma genes for complete root-knot nematode (RKN) resistance in Prunus rootstocks
Nematology 2 (7), 770-770
Presented at the 25th International Nematology Symposium, Herzliya, Israel, 2-7 April 2000
Abstract: The Myrobalan plum (Prunus cerasifera; Prunophora subgenus) is a rootstock species in which the clones P.2175, P.1079 and P.2980 are highly resistant (R) to all tested RKN species including Meloidogyne arenaria (MA), M. incognita (MI), M. javanica (MJ) and also an unclassified population M. sp. Florida (FL) that overcomes the resistance in other commonly used Prunus sources from the Amygdalus subgenus. All three clones carry a single major dominant gene, respectively designated Ma1, Ma2 and Ma3, considered as allelic or closely linked, that confers a high and complete-spectrum resistance (including at least MA, MI, MJ, FL and M. mayaguensis). Bulked segregant analysis associated with the RAPD or AFLP techniques were performed to detect markers linked to the Ma1 gene using segregating intraspecific progenies from P.2175 (Ma1ma1) crossed by several host (H) parents (ma1ma1). Four RAPD and three AFLP markers covering a total distance of approx. 27 cM around the gene were identified and two of them were converted into more reliable SCAR markers with the nearest (SCAL19) located at 3.7 cM from Ma1. SCAL19 has been evaluated for MAS in an European rootstock programme based on Myrobalan plum x Amygdalus (peach, almond or almond-peach) hybrids and proved to be usable reliably in particular to detect any of the Ma genes in segregating progenies involving the resistance sources mentioned above, such as the peach Nemared, the wild peach Prunus davidiana and the almond Alnem. In the Japanese plum (another species of the Prunophora subgenus, interfertile with Myrobalan plum), a new resistance gene (R_jap) has been evidenced from an intraspecific cross J13 (H) x J222 (R) and appears to confer a resistance similar to Ma. One marker elaborated from SCAL19 and located at 10-15 cM from this gene has been obtained and suggests that Ma and R_jap genes could be homologous.
Database assignments for author(s): Daniel Esmenjaud

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne javanica	Cherry-plum (Prunus cerasifera)
	Meloidogyne incognita	Cherry-plum (Prunus cerasifera)
	Meloidogyne arenaria	Cherry-plum (Prunus cerasifera)