Mycoscience (2004) 45, 56-66

Cheng-Ming Tian, Yan-Zhong Shang, Jian-Yun Zhuang, Qi Wang and Makoto Kakishima (2004)
Morphological and molecular phylogenetic analysis of Melampsora species on poplars in China
Mycoscience 45 (1), 56-66
Abstract: Many species of Melampsora on Populus have been reported in China, based on morphological characteristics of both uredial and telial states, and on host species, but their morphology and taxonomy are still poorly defined. In this study, 196 specimens representing Melampsora species on poplars and collected from various areas of China were used for morphological observations. The morphological characteristics of urediniospores and teliospores were examined with light and scanning electron microscopy. The specimens could be classified into five groups based on their morphology. For the sequencing of the nuclear large subunit rDNA (D1/D2), 5.8S rDNA and their internal transcribed spacers, ITS1 and ITS2 region, 54 specimens were selected from the specimens used in morphological observations. These specimens were separated into six clades by phylogenetic analyses of the D1/D2 and ITS regions. Correlations among morphological groups and phylogenetic clades based on these results suggest a revision of these species. In particular, no evidence to discriminate specimens of M. acedioides, M. magnusiana, and M. rostrupii was found from either morphological characteristics or sequence analysis.
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Link to article at publishers website
Database assignments for author(s): Makoto Kakishima, Chengming Tian, Qi Wang

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Melampsora (genus)	Poplar/aspen (Populus)