Mycoscience (2000) 41, 61-78
Kerry O'Donnell, Helgard I. Nirenberg, Takayuki Aoki and Elizabeth Cigelnik (2000)
A multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species
Mycoscience 41 (1), 61-78
Abstract: Phylogenetic relationships within the Gibberella fujikuroi species complex were extended to newly discovered strains using nucleotide characters obtained by sequencing polymerase chain reaction (PCR) amplified DNA from 4 loci used in a previous study [nuclear large subunit 28S rDNA, nuclear ribosomal internal transcribed spacer (ITS) region, mitochondriaal small subunit (mtSSU) ribosomal DNA, and ß-tubulin] together with two newly sampled protein-encoding nuclear genes, translation elongation factor EF-1alpha and calmodulin. Sequences from the ribosomal ITS region were analyzed separately and found to contain of two highly divergent, nonorthologous ITS2 types. Phylogenetic analysis of the individual and combined datasets identified 10 new phylogenetically distinct species distributed among the following three areas: 2 within Asia and 4 within both Africa and South America. Hypotheses of the monophyly of Fusarium subglutinans and its two formae speciales, f. sp. pini and f. sp. ananas, were strongly rejected by a likelihood analysis. Maximum parsimony results further indicate that the protein-encoding nuclear genes provide considerably more phylogenetic signal that the ribosomal genes sequenced. Relative apparent synapomorphy analysis was used to detect long-branch attraction taxa and to obtain a statistical measure of phylogenetic signal in the individual and combined datasets.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Takayuki Aoki
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Fusarium subglutinans | ||||
| Fusarium fujikuroi | ||||
| Fusarium guttiforme |
