Mycologia (2004) 96, 83-101

From Pestinfo-Wiki
Jump to: navigation, search

Bernard Slippers, Pedro W. Crous, Sandra Denman, Teresa A. Coutinho, Brenda D. Wingfield and Michael J. Wingfield (2004)
Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea
Mycologia 96 (1), 83-101
Abstract: Botryosphaeria dothidea is one of the most commonly reported species in a genus of important pathogens of woody plants. This taxon generally is accepted to represent a species complex, and hence its identity remains unclear. Previous studies either have treated B. dothidea as the valid name for B. ribis and B. berengeriana or argued for them to be separate entities. To add to the confusion, no ex-type cultures are available for either B. dothidea or B. ribis. The aim of the present study, therefore, was to recollect and characterize these fungi and designate a set of reference cultures that can be used in future studies. To this end, morphological, cultural and multi-allelic DNA sequence datasets from the rDNA (ITS 1, 5.8S, and ITS 2), ß-tubulin and EF1-alpha genes were used to fully characterize these species. Botryosphaeria dothidea was found to be distinct from B. ribis, while B. berengeriana was retained as synonym of the former name. Furthermore, Fusicoccum aesculi is accepted as anamorph of B. dothidea, while the anamorph of B. ribis is newly described as F. ribis sp. nov. Botryosphaeria ribis could be distinguished from B. parva based on ß-tubulin and EF1-alpha sequence data. A combined phylogeny of the three gene regions used in this study also showed that the genus Botryosphaeria represents two distinct phylogenetic assemblages that correspond to species with Diplodia and Fusicoccum anamorphs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Bernard Slippers, Brenda D. Wingfield, Michael J. Wingfield, Pedro W. Crous, Teresa A. Coutinho, Sandra Denman

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Botryosphaeria dothidea
Neofusicoccum ribis
Neofusicoccum parvum