Molecular Plant-Microbe Interactions (2010) 23, 283-293

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Ken Komatsu, Masayoshi Hashimoto, Johji Ozeki, Yasuyuki Yamaji, Kensaku Maejima, Hiroko Senshu, Misako Himeno, Yukari Okano, Satoshi Kagiwada and Shigetou Namba (2010)
Viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways
Molecular Plant-Microbe Interactions 23 (3), 283-293
Abstract: Resistant plants respond rapidly to invading avirulent plant viruses by triggering a hypersensitive response (HR). An HR is accompanied by a restraint of virus multiplication and programmed cell death (PCD), both of which have been observed in systemic necrosis triggered by a successful viral infection. Here, we analyzed signaling pathways underlying the HR in resistance genotype plants and those leading to systemic necrosis. We show that systemic necrosis in Nicotiana benthamiana, induced by Plantago asiatica mosaic virus (PlAMV) infection, was associated with PCD, biochemical features, and gene expression patterns that are characteristic of HR. The induction of necrosis caused by PlAMV infection was dependent on SGT1, RAR1, and the downstream mitogen-activated protein kinase (MAPK) cascade involving MAPKKK-alpha and MEK2. However, although SGT1 and RAR1 silencing led to an increased accumulation of PlAMV, silencing of the MAPKKK-alpha-MEK2 cascade did not. This observation indicates that viral multiplication is partly restrained even in systemic necrosis induced by viral infection, and that this restraint requires SGT1 and RAR1 but not the MAPKKK-alpha-MEK2 cascade. Similarly, although both SGT1 and MAPKKK-alpha were essential for the Rx-mediated HR to Potato virus X (PVX), SGT1 but not MAPKKK-alpha was involved in the restraint of PVX multiplication. These results suggest that systemic necrosis and HR consist of PCD and a restraint of virus multiplication, and that the latter is induced through unknown pathways independent from the former.
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Database assignments for author(s): Shigetou Namba

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Plantago asiatica mosaic virus