Molecular Plant-Microbe Interactions (2000) 13, 366-373

Tuula Mäki-Valkama, Jari P.T. Valkonen, Jan F. Kreuze and Eija Pehu (2000)
Transgenic resistance to PVY(^O) associated with post-transcriptional silencing of P1 transgene is overcome by PVY(^N) strains that carry highly homologous P1 sequences and recover transgene expression at infection
Molecular Plant-Microbe Interactions 13 (4), 366-373
Abstract: Resistance to Potato virus Y (PVY) has been obtained in our previous studies through expression of the PVY P1 gene in sense or antisense orientation in potato cv. Pito. In the present study, the mechanism and strain specificity of the resistance were analyzed. Several features including low steady-state P1 mRNA expression in the resistant P1 plants indicated that resistance was based on post-transcriptional gene silencing (PTGS). Resistance was specific to PVY^O isolates, the PVY strain group from which the P1 transgene was derived. However, according to group analyses, there was no distinguishing characteristic between the PVY^O and PVY^N strains P1 gene sequences. Therefore, the ability of the PVY^N strains to overcome resistance could not be explained solely based on their P1 gene sequences. Infection with PVY^N of the PVY^O-resistant transgenic lines led to a recovery of expression of the P1 transgene. These data suggested that factors other than sequence homology are required in determination of the resistance specificity.
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Link to article at publishers website
Database assignments for author(s): Jari P.T. Valkonen, Jan F. Kreuze

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Potato virus Y