Molecular Plant-Microbe Interactions (1998) 11, 1130-1135

Shaojian Gao and Donald L. Nuss (1998)
Mutagenesis of putative acylation sites alters function, localization, and accumulation of a gialpha subunit of the chestnut blight fungus Cryphonectria parasitica
Molecular Plant-Microbe Interactions 11 (11), 1130-1135
Abstract: Targeted disruption of cpg-1, a gene encoding the G protein Gialpha subunit, CPG-1, in the chestnut blight fungus, Cryphonectria parasitica, results in reduced mycelial growth, reduced orange pigmentation, loss of virulence, loss of asexual sporulation, and female infertility. We report the development of a complementation system for cpg-1 null mutants and its use to evaluate the in vivo consequences of mutating conserved putative CPG-1 myristoylation (G2) and palmitoylation (C3) sites. Independent mutations of the two putative acylation sites differentially altered complex fungal biological processes, including virulence, and modified CPG-1 membrane association. Results of combined Northern (RNA) and Western (immunoblot) analysis also indicated a role for lipid modification in post-transcriptional regulation of CPG-1 accumulation.
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Link to article at publishers website
Database assignments for author(s): Donald L. Nuss

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cryphonectria parasitica