Molecular Ecology Resources (2009) 9, 958-960

Dina M. Fonseca, Kenli Okada and Laura D. Kramer (2009)
Microsatellite loci for the white-dotted mosquito (Culex restuans), a principal vector of West Nile virus in North America
Molecular Ecology Resources 9 (3), 958-960
Abstract: We characterized the first microsatellite loci in the white-dotted mosquito, Culex restuans, a critical early spring West Nile virus vector. An enrichment protocol yielded 960 positive clones of which we sequenced 300. We designed primers to amplify 29 unique di-, tri- and tetranucleotide microsatellites and chose 17 that amplified consistently across populations and were polymorphic. We developed three multiplex primer combinations for all 17 loci. A survey of 44 individuals revealed two to 20 alleles across loci, and expected heterozygosity ranging from 0.17 to 0.89. These markers will allow examination of the life history of this mysterious early season encephalitis vector.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Dina M. Fonseca, Laura D. Kramer

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex restuans