Molecular Ecology Notes (2007) 7, 1363-1365

X. Giresse, D. Tourvieille De Labrouhe, S. Richard-Cervera and F. Delmotte (2007)
Twelve polymorphic expressed sequence tags-derived markers for Plasmopara halstedii, the causal agent of sunflower downy mildew
Molecular Ecology Notes 7 (6), 1363-1365
Abstract: Twelve expressed sequence tags-derived markers were isolated from Plasmopara halstedii (Oomycetes), the causal agent of sunflower downy mildew. A total of 25 single nucleotide polymorphisms and five indels were detected by single-strand conformation polymorphism analysis and developed for high-throughput genotyping of 32 isolates. There was a high level of genetic diversity (H_E = 0.484). Observed heterozygosity ranged from 0 to 0.143 indicating that P. halstedii is probably a selfing species. These markers were also useful in detecting significant genetic variations among French populations (F_ST = 0.193) and between French and Russian populations (F_ST = 0.23). Cross-amplification tests on three closely related species indicated that no loci amplified in other Oomycete species.
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Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plasmopara halstedii		France