Molecular Ecology Notes (2004) 4, 80-82

M.F. Zapater, A. Rakotonantoandro, S. Cohen and J. Carlier (2004)
Polymerase chain reaction-restriction fragment length polymorphism markers for the fungal banana pathogen Mycosphaerella fijiensis
Molecular Ecology Notes 4 (1), 80-82
Abstract: Polymerase chain reaction-restriction fragment length polymorphism markers were developed to study populations of the fungal banana pathogen Mycosphaerella fijiensis. Twelve markers were defined, 11 in anonymous and single-copy nuclear DNA sequences and one in the internal transcribed spacer and 5.8S rDNA sequence. The polymerase chain reaction products obtained with locus-specific primer pairs were digested with restriction enzymes to reveal polymorphism. Between five and 12 markers were polymorph in M. fijiensis populations from different geographical origins (Papua New Guinea, the Philippines, Cameroon and Latin America). The mean of allele number and gene diversity (expected heterozygosity) per locus in the different geographical populations ranged between 1.4 and 2.7 and 0.17 and 0.45, respectively.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pseudocercospora fijiensis	Banana/plantain (Musa)	Papua New Guinea
	Pseudocercospora fijiensis	Banana/plantain (Musa)	Philippines
	Pseudocercospora fijiensis	Banana/plantain (Musa)	Cameroon